T cells accumulate in non-diabetic islets during ageing
نویسندگان
چکیده
Abstract Background The resident immune population of pancreatic islets has roles in islet development, beta cell physiology, and the pathology diabetes. These have largely been attributed to macrophages, comprising 90% cells (in absence autoimmunity), and, case type 1 diabetes, infiltrating autoreactive T cells. In adipose, tissue-resident recruited B implicated development insulin resistance during diet-induced obesity ageing, but whether this is paralleled not known. Here, we investigated non-macrophage component isolated from C57BL/6 J male mice ageing (3 24 months age) following similar weight gain achieved by 12 weeks 60% high fat diet. Immune were also examined flow cytometry cadaveric non-diabetic human islets. Results comprised 2.7 ± 1.3% total mouse islets, 2.3 1.7% 3-month old on standard diet, each approximately 2–4% compartment, 0.1% A amount present majority expressed αβ receptor, CD8-positive, CD4-positive, regulatory cells, with a minor γδ Interestingly, number increased linearly (R 2 = 0.9902) age 0.10 0.05% months) 0.38 0.11% (24 This increase was uncoupled body weight, phenocopied degree induced diet mice. Conclusions study reveals that are part normal accumulate weight-independent manner. Though only small subset within may play role physiology ageing.
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ژورنال
عنوان ژورنال: Immunity & Ageing
سال: 2021
ISSN: ['1742-4933']
DOI: https://doi.org/10.1186/s12979-021-00221-4